Effects of cholinergic agents on rat liver plasma membrane guanylate cyclase

Evidence for Histidine Residues on Plasma Membrane Phosphatidate Phosphohydrolase from Rat Liver

Objective(s) Phosphatidate phosphohydrolase (PAP) catalyzes the dephosphorylation of phosphatidic acid to yield Pi and  diacylglycerol. Two different forms of PAP in rat hepatocyte have been reported. PAP1 is located in cytosolic and microsomal fractions and participates in the synthesis of triacylglycerols, phosphatidylcholine, and phosphatidylethanolamine, whereas the other form of phosphati...

Transient receptor coupling in the activation of rat liver plasma-membrane adenylate cyclase by glucagon.

The glucagon and fluoride sensitive adenyl cyclase in plasma membrane of rat liver.

Certain polypeptide hormones and catecholamines act to stimulate adenyl cyclase and then increase the formation of adenosine 3',5'-cyclic monophosphate (cyclic AMP). Cyclic AMP activates protein kinases which in turn affect the activities of certain enzymes and properties of some proteins. This process is thought to be important as a mechanism of hormone actions. The localization of adenyl cycl...

Effects of cations on guanylate cyclase of sea urchin sperm.

Mn2+ and to some degree Fe2+, but not Mg+, Ca2+, ba2+, Sr2+, Co2+, Ni2+, La3+, or Fe3+ were able to serve as effective metal cofactors for sea urchin sperm guanylate cyclase. The apparent Michaelis constant for Mn2+ in the presence of 0.25 mM MnGTP was 0.23 mM. In the presence of a fixed free mn2+ concentration, variation in mngTP resulted in sigmoid velocity-substrate plots and in reciprocal p...

The Effects of Nitroxyl (HNO) on Soluble Guanylate Cyclase Activity

It has been previously proposed that nitric oxide (NO) is the only biologically relevant nitrogen oxide capable of activating the enzyme soluble guanylate cyclase (sGC). However, recent reports implicate HNO as another possible activator of sGC. Herein, we examine the affect of HNO donors on the activity of purified bovine lung sGC and find that, indeed, HNO is capable of activating this enzyme...